PERTANIKA JOURNAL OF SCIENCE AND TECHNOLOGY

The mineralization of urea fertilizer significantly impacts nitrogen movement in the soil. An incubation study was done on a lab scale basis to examine nitrogen dynamics in soil inoculated with plant growth-promoting bacteria (PGPB) supplemented with varying levels of nitrogen fertilizer in the form of urea (0% N, 25% N, 50% N, 75% N, and 100% N). In the present experiment, sandy clay loam soil was used and incubated for four weeks, and the concentrations of NH₄⁺‒N and NO₃–‒N were monitored using the destructive method (Kjeldahl) to determine the mineralization rate of urea. Results showed higher NH₄⁺‒N (11.880 mg/kg mineralized with UPMRB9N50 treatment) and NO₃–‒N (20.060 mg/kg mineralized with UPMRB9N50 treatment) concentrations in the bacteria-treated soil compared to the uninoculated control. Urea-N remains higher (0.0353% and 0.0253% from UPMRB9N50 treatment in the first and second weeks, respectively) in bacteria-treated soil during the first two weeks, then gradually becomes zero towards the end of the observing period. Nitrogen (N) leaching loss was lower in bacterial inoculated soil compared to the control, and the leaching loss of N was greater with the increased N fertilizer rates. Cumulative N leaching loss is higher (29.797 mg/kg) in 100% N-treated soil than in other treatments. The findings observed that the beneficial bacteria could enhance the N mineralization to make the nutrient available for the crop while, at the same time, reducing leaching losses of fertilizer when supplied with a minimum amount of chemical fertilizer, thereby saving the input cost and protecting the environment.

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